Comparison

CANX Antibody

Item no. PRS-42-774-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, IF, IHC, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Caprine
Conjugate/Tag Unconjugated
NCBI CANX
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias CANX,calnexin,CNX,FLJ26570,IP90,P90,major histocompatibility complex class I antigen-binding protein p88
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
By Type
Other
Modifications
None
Protein Gi #
10716563
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1
Positive Control 2
Positive Control 3
Positive Control 4
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7; 271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml


Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Homology
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Manufacturer - Specificity
Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
Immunogen
The immunogen for this antibody is: C-SKTPELNLDQFHDKT
Clonality
Polyclonal
NCBI Official Name
calnexin
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
available

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