PLA2G4F Antibody

Primary Antibodies

Aliquot and store at -20˚ C. Minimize freezing and thawing.

None

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

500 ug/mL

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

The immunogen for this antibody is: C-QTAEEKAFGDFVINR

phospholipase A2, group IVF

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.