Comparison

MED12 Antibody

Item no. PRS-43-315-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications ELISA
Specific against other
Host Caprine
Conjugate/Tag Unconjugated
Citations Xu X, Zhou H, Boyer TG.Mediator is a transducer of amyloid-precursor-protein-dependent nuclear signalling.
NCBI MED12
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias MED12, mediator complex subunit 12, ARC240, CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230, CAG repeat protein 45, OPA-containing protein, OTTHUMP00000023490, OTTHUMP00000023493, activator-recruited cofactor 240 kDa component, human opposite pai
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Manufacturer - Research Area
Immunology
By Type
Immunology
Modifications
None
Protein Gi #
110347429
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_005111.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Homology
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Pig, Cow
Immunogen
The immunogen for this antibody is: C-NPTLVDPTRHLQQR
Clonality
Polyclonal
NCBI Official Name
mediator complex subunit 12
NCBI Organism
Homo Sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Manufacturer - Additional Names
MED12, mediator complex subunit 12, ARC240, CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230, CAG repeat protein 45, OPA-containing protein, OTTHUMP00000023490, OTTHUMP00000023493, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA polymerase II transcription subunit 12, mediator of RNA polymerase II transcription, subunit 12 homolog, thyroid hormone receptor-associated protein complex 230 kDa component, thyroid hormone receptor-associated protein, 230 kDa subunit, trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit), trinucleotide repeat-containing gene 11 protein

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
available

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