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PAI-1 Antibody

PAI-1 Antibody

Item no.	PRS-45-142-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Food Safety Proteins Antibodies Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Senescence Primary Antibodies By Organ Lung Cancer Breast Cancer Pancreatic Cancer
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC, ELISA
Specific against	Human (Homo sapiens)
Host	Caprine
Conjugate/Tag	Unconjugated
Citations	Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E. Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol. 2003 Oct;15(10):1207-18.
NCBI	SERPINE1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor-1
Available
Manufacturer - Applications	Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6ug/ml.A customer successfully used this product on Human Breast cancer.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and store at -20°C. Minimize freezing and thawing.
Manufacturer - Research Area	Signal Transduction , Obesity
Protein Gi #	10835159
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1	PRS-1304-0.1mg
Positive Control 2	PRS-1309-0.1mg
Concentration	500 ug/mL
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Homology	Expected Species Reactivity based on sequence homology: Dog
Immunogen	The immunogen for this antibody is: C-GFKIDDKGMAPALRH
Clonality	Polyclonal
NCBI Official Name	serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/4/2025

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