Comparison

Mouse Wee1 Antibody

Item no. PRS-60-265-400ul
Manufacturer ProSci
Amount 400 ul
Category
Type Antibody Polyclonal
Format Liquid
Applications WB
Specific against Human (Homo sapiens)
Host Rabbit
Conjugate/Tag Unconjugated
Citations Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010) :
Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008)
Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007)
Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006)
NCBI Wee1
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Wee1-like protein kinase,Wee1A kinase,Wee1
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Manufacturer - Isotype
Rabbit Ig
Shipping Temperature
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
72 kDa
Manufacturer - Research Area
Cell Cycle
Background
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
By Type
Cell Cycle
Modifications
None
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Application Note
For WB starting dilution is: 1:1000
Homology
Predicted species reactivity based on immunogen sequence: Rat
Immunogen
This Mouse Wee1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of mouse Wee1.
Clonality
Polyclonal
NCBI Official Name
Wee1-like protein kinase
NCBI Organism
Mus musculus
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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