USP5 Antibody

For WB starting dilution is: 1:1000

blue ice or room temperature

96 kDa

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Supplied in PBS with 0.09% (W/V) sodium azide.

This USP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-514 amino acids from the C-terminal region of human USP5.

Ubiquitin carboxyl-terminal hydrolase 5

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.