IL-20 Antibody

Primary Antibodies, Antibody Pairs

IL-20 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

None

Anti-hIL-20 specific antibody was purified by affinity chromatography employing immobilized hIL-20 matrix.

ELISA:
To detect hIL-20 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-20.
Sandwich:
To detect hIL-20 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-20 (38-133) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.
Western Blot:
To detect hIL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.