IL-6 Antibody

ELISA, WB, Neutralization Assay

IL-6 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

Interleukin 6 (IL-6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL-6 has also been implicated in regulating adipose mass.

None

Anti-Rat IL-6 specific antibody was purified by affinity chromatography employing immobilized Rat IL-6 matrix.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-6 (0.5 ng/mL), a concentration of 0.30 0.50 μ g/mL of this antibody is required.
ELISA:
To detect Rat IL-6 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-6.
Sandwich:
To detect Rat IL-6 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-6 (XP-5195Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.
Western Blot:
To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Rattus norvegicustus norvegicus

Centrifuge vial prior to opening.