IL-4 Antibody [1.3_4F11-2G3]

ELISA, WB, Neutralization Assay

IL-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

None

This IgG1k antibody was purified on a Protein A affinity column.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.0 ng/mL), a concentration of 6.0-8.5 μ g/mL of this antibody is required.ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 0.5-1.0 μ g/mL of this antibody will detect at least 2000 pg/mL of recombinant human IL-4 when used with our biotinylated antigen affinity purified anti-human IL-4 (XP-5188Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μ g/mL.
Western Blot:
To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.50 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.