FGF-basic Antibody [20B242.2]

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with our biotinylated antigen affinity purified anti-human FGF-basic (XP-5137Bt) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL.

Western blot:

To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.

FGF-basic lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

261260095

The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hFGF-basic as the immunizing antigen.

fibroblast growth factor 2 (basic)

This product is for research use only.