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Progesterone Receptor (phospho Ser190) Antibody [1154]

Progesterone Receptor (phospho Ser190) Antibody [1154]

Item no.	PRS-XPS-1023-0.1mL
Manufacturer	ProSci
Amount	0.1 mL
Category	Oncology Food Safety Hormones Antibodies By Cell Type Carcinoma Sarcoma Immunohistochemistry Western Blot Primary Antibodies By Organ Breast Cancer
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC
Clone	1154
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
Citations	Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P., "Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody," Mol. Endocrinol., 14 (2000) 52 - 65. Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C., "Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion," Endocrinology 144 (2003) 5650 -5657.
NCBI	PGR
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Applications	As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
Shipping Temperature	blue ice or room temperature
Storage Conditions	For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Molecular Weight	90/120
Manufacturer - Research Area	Signal Transduction , Growth Factor , Phospho-Specific
Background	There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Modifications	Phospho-Specific
Protein Gi #	90110048
Purification	Protein G purified
Immunogen	Progesterone receptor (Ser190) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor.
Clonality	Monoclonal
NCBI Official Name	progesterone receptor
NCBI Organism	Homo sapiens
Disclaimer	This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mL

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Delivery expected until 9/4/2025

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