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Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)

Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)

Item no.

SGE-CFT45325

Manufacturer

Cosmobio

Amount

1 ml

Category

Type

Antibody Monoclonal

Applications

Clone

H25, H53, B51

Specific against

Human (Homo sapiens)

Host

Rat

Conjugate/Tag

Fluorescein Isothiocyanate / Texas RedTM

Citations

Samar M. Said, Mary E.Fidler, Anthony M. Valeri, Brooke McCann, Wade Fiedler, Lynn D.Cornell, Mariam Priya Alexander, Ahmed M. Alkhunaizi, Anne Sullivan, Carl H. Cramer, Marie C. Hogan, Samih H. Nasr, Kidney Int Rep. 2017 Jan;2(1):44-52. PMID:
Malone AF, Funk SD, Alhamad T, Miner JH., Pediatr Nephrol. 2017 Jun;32(6):997-1003. PMID: 28013382
Nishizawa Y, Takei T, Miyaoka T, Kamei D, Mochizuki T, Nitta K., J Obstet Gynaecol Res. 2016 Mar;42(3):331-5. PMID: 26628290
Nozu K, Vorechovsky I, Kaito H, Fu XJ, Nakanishi K, Hashimura Y, Hashimoto F, Kamei K, Ito S, Kaku Y, Imasawa T, Ushijima K, Shimizu J, Makita Y, Konomoto T, Yoshikawa N, Iijima K., Clin J Am Soc Nephrol. 2014 Nov 7;9(11):1958-64. PMID: 25183659
Matsubara S, Ueda Y, Takahashi H, Nagai T, Kuwata T, Muto S, Yamaguchi T, Takizawa T, Suzuki M., J Obstet Gynaecol Res. 2009 Dec;35(6):1109-14. PMID: 20144175
Patey-Mariaud de Serre N, Garfa M, Bessieres B, Noel LH, Knebelmann B., Kidney Int. 2007 Aug;72(4):512-6. PMID: 17554254
Kharrat M, Makni S, Makni K, Kammoun K, Charfeddine K, Azaeiz H, Jarraya F, Ben Hmida M, Gubler MC, Ayadi H, Hachicha J., Saudi J Kidney Dis Transpl. 2006 Sep;17(3):320-5. PMID: 16970251
Zhang SY, Marlier A, Gribouval O, Gilbert T, Heidet L, Antignac C, Gubler MC., Kidney Int. 2004 Sep;66(3):945-54. PMID: 15327385
Heidet L, Bongers EM, Sich M, Zhang SY, Loirat C, Meyrier A, Broyer M, Landthaler G, Faller B, Sado Y, Knoers NV, Gubler MC., Am J Pathol. 2003 Jul;163(1):145-55. PMID: 12819019;Borza DB, Bondar O, Ninomiya Y, Sado Y, Naito I, Todd P, Hudson BG., J Biol Chem. 2001 Jul 27;276(30):28532-40. PMID: 11375996
Kagawa M, Kishiro Y, Naito I, Nemoto T, Nakanishi H, Ninomiya Y, Sado Y., Nephrol Dial Transplant. 1997 Jun;12(6):1238-41. PMID: 9198058
Naito I, Kawai S, Nomura S, Sado Y, Osawa G., Kidney Int. 1996 Jul;50(1):304-11. PMID: 8807602
Ninomiya Y, Kagawa M, Iyama K, Naito I, Kishiro Y, Seyer JM, Sugimoto M, Oohashi T, Sado Y., J Cell Biol. 1995 Sep;130(5):1219-29. PMID: 7657706
Yoshioka K, Hino S, Takemura T, Maki S, Wieslander J, Takekoshi Y, Makino H, Kagawa M, Sado Y, Kashtan CE., Am J Pathol. 1994 May;144(5):986-96. PMID: 8178947

ECLASS 10.1

42030590

ECLASS 11.0

42030590

UNSPSC

12352203

Available

Manufacturer - Category

Antibodies/Primary Antibodies/Cell Biology/Cytoskeleton; Research Area/Cell Biology/Cytoskeleton/Primary Antibodies; Research Area/Cell Biology/Extracellular Matrix/Primary Antibodies; Research Area/Cell Biology/Signal Transduction/Primary Antibodies; Antibodies/Primary Antibodies/Cell Biology/Extracellular Matrix; Antibodies/Primary Antibodies/Cell Biology/Signal Transduction; Research Area/Disease/Alport Syndrome/Primary Antibodies; Research Area/Disease/Goodpastures Syndrome/Primary Antibodies; Antibodies/Primary Antibodies/Disease/Alport Syndrome; Antibodies/Primary Antibodies/Disease/Goodpastures Syndrome; Research Area/Organs & Organ Systems/Kidney/Primary Antibodies; Antibodies/Primary Antibodies/Organs & Organ Systems/Kidney

Maker

SGE

Antigen/Source

clone H53 is specific to imperfection III of Collagen IV alpha5(IV) Chain,
clone B51 is specific to NC1 domain of Collagen IV alpha5(IV) Chain,
clone H25 is specific to imperfection XIII of Collagen IV alpha2(IV) Chain

Short Description

This immuno-staining cocktail of three differentially labeled collagen isotype-specific rat monoclonal antibodies helps in analyses of renal and skin biopsies to distinguish Alport syndrome from unaffected individuals.\\Two clones (H53, B51) labeled with FITC reveal the diagnostic collagen α5(IV) while a third (H25), labeled with Texas Red, detects collagen α2(IV), revealing basement membrane structure.

Description

Check out our Human Collagen IVα Rat Monoclonal Antibodies Dashboardfor more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV.

Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen α 5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti-Collagen 4 Cocktail mAb (cat no. SGE-CFT45325) is a cocktail of three different rat mAbs for easy and rapid staining of human renal and skin biopsy sections to distinguish Alport syndrome from normal tissue. It comprises two different FITC-conjugated mAbs (clones H53 and B51) that reveal the Alport-affected α 5(IV) chain in glomerular, tubular and Bowman's capsular BMs and an internal positive control Texas Red-conjugated mAb (clone H25) that targets α 2(IV) to reveal endothelial basement membrane structure

Product specifications

Specification	Detail
Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)
Clonality	Monoclonal
Immunogen	Synthetic peptide of alpha5 chain and alpha2 chain
Intended use	Staining of human cryostat sections by direct immunofluorescence (No acid-urea treatment is necessary.)
Fraction	Affinity Purified
Preservative	0.1% NaN3 in LIQ

Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

SGE-CFT45325.pdf

SGE-CFT45325-datasheet.pdf

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Amount: 1 ml

available

Delivery expected until 9/11/2025

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