EnzyFluo NADP/NADPH Assay Kit

Application

• For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features

• Sensitive and accurate. Detection limit of 0.01 ?M and linearity up to 1 ?M NADP+/NADPH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• FL530/585nm

Samples

• Cell, tissue extracts etc

Species

• All

Size

• 100 tests

Detection Limit

• 0.01 ?M

Shelf Life

• 6 months

More Details

• Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP⁺/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP⁺/NADPH concentration are very desirable. BioAssay Systems EnzyFluo NADP⁺/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ?ex/em = 530/585 nm, is proportional to the NADP⁺/NADPH concentration in the sample. This assay is highly specific for NADP⁺/NADPH and with minimal interference (< 1%) by NAD⁺/NADH. Our assay is a convenient method to measure NADP⁺, NADPH and their ratio.