Amylin 3 Receptor (AMY3) Stable Cell Line

8) Stable Cell Lines / 12.1) Cell Lines, ACTOne Assay on G-Protein Coupled Receptors (GPCRs), HEK293

This cell line expresses a recombinant human RAMP3. The parental cell line is Calcitonin Receptor (CALCR) ACTOne Stable Cell Line (CL-01-CALCR) that is HEK-293-CNG cell line that expresses a recombinant human CALCR. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader.