Comparison

Assisted reproductive technologies

 

Introduction

In recent years, the number of genetically engineered mice being produced has increased dramatically. Moreover, the rapid progress in the development of genome-editing (TALEN and CRISPR/Cas9) techniques for molecular biology reserach has been remarkable, so much so that a genetically engineered mouse strain can be produced easily in a few months. Production has been supported by reproductive engineering techniques such as In vitro fertilisation, embryo and sperm cryopreservation and embryo transfer techniques. Such techniques have become invaluable peripheral technologies and their use is expanding rapidly. 

Fig. 1: Lebenszyklus einer Maus bei Nutzung von FERTIUP® und CARD MEDIUM®

Overview

To support your research we offer several reagents and disposable items (general labware) for In vitro fertilisation, In vitro culture, cryopreservation and In vitro manipulation.

CARD MEDIUM® and FERTIUP®

Sperm cryopreservation is a practical and cost-effective method of archiving mouse strains in the laboratory. FERTIUP® and CARD MEDIUM® are high-performance media for mouse sperm cryopreservation, sperm preincubation and In vitro fertilization (IVF). 


When used in combination, FERTIUP® and CARD MEDIUM® provide a high and stable fertilization rate using frozen-thawed mouse sperm. This will allow you to reproduce mice whenever you need them.
The FERTIUP® sperm cryoprotectant and sperm preincubation medium greatly improve fertilization rates of frozen-thawed sperm in C57BL/6 and 129 Mice strains, from less than 10% (using conventional methods) to more than 30%. 
In addition, CARD MEDIUM® enhances the fertility rate to over 80%. FERTIUP® and CARD MEDIUM® have no reproduction toxicity of any kind. 


The newly developed system for sperm cryopreservation and IVF using FERTIUP® and CARD MEDIUM® provides superior support for the safe and reliable preservation of your valuable mouse strains.

No.StrainsMethodNumber of
oocytet donors
Number of
inseminated oocytes
Average number of
ovulated oocytes/female
No. of
fertilized eggs
Fertilization rate (%)
1 C57BL/6J eCG
Product
10
10
270
1072
27.7±5.4
107.2±22.7
267
963
96.4±3.2
89.8±3.7
2 BALB/c ByJ eCG
Product
10
10
293
903
29.3±8.9
90.3±14.5
213
680
73.4±11.3
76.4±9.0
3 C3H/HeJ eCG
Product
10
10
286
520
28.6±8.4
52.0±18.1
251
446
87.8±22.6
85.8±15.5
4 DBA/2J eCG
Product
10
10
225
688
22.5±7.8
68.8±13.6
194
592
86.2±11.0
86.0±4.5
5 FVB/NJ eCG
Product
10
10
168
256
16.8±3.3
25.6±5.9
159
239
94.6±6.7
93.3±6.6
6 CD1 eCG
Product
10
10
202
337
20.2±8.0
33.7±9.9
146
269
72.3±16.0
79.8±19.6

Fig. 2:

  • 7.5 IU of CARD HyperOva® or PMSG was injected to four weeks old female mice, then 7.5 IU of hCG was administered 48 hours later.
    Thereafter all ova obtained 17 hours after the hCG injection were collected.
  • The In vitro fertilization rate is defined as the number of 2-cell embryos divided by the total number of ova (mean±SD).
  • Data cited from a paper published by Prof. Naomi Nakagata and Toru Takeo (Center for Animal Resources and development, Kumamoto University)
Fig. 3: Fertilization rates using FERTIUP® Preincubation Medium and CARD MEDIUM® for fresh and frozen sperm in various strains.
Fig. 4: Fertilization rates using frozen-thawed sperm with FERTIUP® and with FERTIUP® and CARD MEDIUM®

CARD HyperOva® the superovulation reagent

Reduction in experimental animal use (Contribution to 3Rs):
Using CARD HyperOva® enables us to reduce the number of female mice used for ovum collection by 25 - 30 %.

Optimization of genetically modified mouse creation and preservation:
More ova can be obtained from less female mice using CARD HyperOva® than following the conventional method, which will facilitate In vitro fertilization and embryo transfer.

Application to valuable genetically modified mice:
Using CARD HyperOva® on your valuable genetically modified mice enables you to obtain a lot of ova from small number of females, thus enabling the large-scale preparation of embryos.

Periphere products

Additionally to reagents regarding reproductive engineering techniques we offer several instruments. For example a transportation kit for the cauda epididymides or straws to execute In vitro fertilisation steps.

Relevant products for the assisted reproductive technologies

Items 1 to 25 of 78 total

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Name PDF Type Clone Specific against Appl. Host Item no. Amount Price
Sperm diluent for in vitro fertilization Download instruction sheet Cells, Tissue Culture other CSR-CK026

Cosmobio
25 ML Request
Compare
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium (KYD-­001-­05-­EX & KYD-­002-­05-­EX) Cells, Tissue Culture other KYD-SET-01

Cosmobio
1 SET Request
Compare
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card HyperOva (KYD-­001-­05-­EX & KYD-­002-­05-­EX & KYD-­010-­EX) Cells, Tissue Culture other KYD-SET-02

Cosmobio
1 SET Request
Compare
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card Medium + Card HyperOva (KYD-­001-­05-­EX & KYD-­002-­05-­EX & KYD-­003-­EX & KYD-­010-­EX) Cells, Tissue Culture other KYD-SET-03

Cosmobio
1 SET Request
Compare
FERTIUP Mouse Sperm Preincubation Medium + Card Medium + Card HyperOva (KYD-­002-­05-­EX & KYD-­003-­EX & KYD-­010-­EX) Cells, Tissue Culture other KYD-SET-04

Cosmobio
1 SET Request
Compare
Cold storage solution for cauda epididymides Download instruction sheet Cells, Tissue Culture other KYD-007-EX

Cosmobio
1 SET Request
Compare
CARD MEDIUM((R)) Download instruction sheet Cells, Tissue Culture other KYD-003-EX

Cosmobio
1 KIT Request
Compare
mHTF Cells, Tissue Culture other KYD-008-02-EX

Cosmobio
2 ML Request
Compare
mHTF Cells, Tissue Culture other KYD-008-05-EX-X3

Cosmobio
3*5 ML Request
Compare
mHTF Cells, Tissue Culture other KYD-008-05-EX

Cosmobio
5 ML Request
Compare
mHTF Cells, Tissue Culture other KYD-008-02-EX-X5

Cosmobio
5*2 ML Request
Compare
FERTIUP Mouse Sperm Cryoprotectant: CPA Download instruction sheet Cells, Tissue Culture other KYD-001-05-EX

Cosmobio
0.5 ML Request
Compare
FERTIUP Mouse Sperm Cryoprotectant: CPA Download instruction sheet Cells, Tissue Culture other KYD-001-EX

Cosmobio
1 ML Request
Compare
FERTIUP Mouse Sperm Cryoprotectant: CPA x5 Download instruction sheet Cells, Tissue Culture other KYD-001-05-EX-X5

Cosmobio
5*0.5 ML Request
Compare
FERTIUP Mouse Sperm Cryoprotectant: CPA x5 Download instruction sheet Cells, Tissue Culture other KYD-001-EX-X5

Cosmobio
5*1 ML Request
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FERTIUP Mouse Sperm Preincubation Medium: PM Download instruction sheet Cells, Tissue Culture other KYD-002-05-EX

Cosmobio
0.5 ML Request
Compare
FERTIUP Mouse Sperm Preincubation Medium: PM Download instruction sheet Cells, Tissue Culture other KYD-002-EX

Cosmobio
1 ML Request
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FERTIUP Mouse Sperm Preincubation Medium: PM x5 Download instruction sheet Cells, Tissue Culture other KYD-002-05-EX-X5

Cosmobio
5*0.5 ML Request
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FERTIUP Mouse Sperm Preincubation Medium: PM x5 Download instruction sheet Cells, Tissue Culture other KYD-002-EX-X5

Cosmobio
5*1 ML Request
Compare
FERTIUP PM 0.5ML-­CARD MEDIUM set Download instruction sheet Cells, Tissue Culture other KYD-005-EX

Cosmobio
1 SET Request
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FERTIUP PM 1ML-­CARD MEDIUM set Download instruction sheet Cells, Tissue Culture other KYD-004-EX

Cosmobio
1 SET Request
Compare
CARD HyperOva Download instruction sheet Bio-active substances other KYD-010-EX

Cosmobio
1 ML Request
Compare
CARD HyperOva Download instruction sheet Bio-active substances other KYD-010-EX-X5

Cosmobio
5*1 ML Request
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CARD HyperOva Download instruction sheet Bio-active substances other KYD-010-06-EX

Cosmobio
0.6ML Request
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CARD HyperOva Download instruction sheet Bio-active substances other KYD-010-06-EX-X5

Cosmobio
5*0.6ML Request
Compare
Name Price
Sperm diluent for in vitro fertilization Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 25 ML
Available
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium (KYD-­001-­05-­EX & KYD-­002-­05-­EX) Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card HyperOva (KYD-­001-­05-­EX & KYD-­002-­05-­EX & KYD-­010-­EX) Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card Medium + Card HyperOva (KYD-­001-­05-­EX & KYD-­002-­05-­EX & KYD-­003-­EX & KYD-­010-­EX) Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
FERTIUP Mouse Sperm Preincubation Medium + Card Medium + Card HyperOva (KYD-­002-­05-­EX & KYD-­003-­EX & KYD-­010-­EX) Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
Cold storage solution for cauda epididymides Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
CARD MEDIUM((R)) Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 KIT
Available
mHTF Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 2 ML
Available
mHTF Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 3*5 ML
Available
mHTF Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5 ML
Available
mHTF Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*2 ML
Available
FERTIUP Mouse Sperm Cryoprotectant: CPA Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 0.5 ML
Available
FERTIUP Mouse Sperm Cryoprotectant: CPA Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 ML
Available
FERTIUP Mouse Sperm Cryoprotectant: CPA x5 Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*0.5 ML
Available
FERTIUP Mouse Sperm Cryoprotectant: CPA x5 Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*1 ML
Available
FERTIUP Mouse Sperm Preincubation Medium: PM Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 0.5 ML
Available
FERTIUP Mouse Sperm Preincubation Medium: PM Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 ML
Available
FERTIUP Mouse Sperm Preincubation Medium: PM x5 Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*0.5 ML
Available
FERTIUP Mouse Sperm Preincubation Medium: PM x5 Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*1 ML
Available
FERTIUP PM 0.5ML-­CARD MEDIUM set Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
FERTIUP PM 1ML-­CARD MEDIUM set Request
Type Cells, Tissue Culture
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 SET
Available
CARD HyperOva Request
Type Bio-active substances
Clone
Specific against other
Appl.
Host
Item no.
Amount 1 ML
Available
CARD HyperOva Request
Type Bio-active substances
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*1 ML
Available
CARD HyperOva Request
Type Bio-active substances
Clone
Specific against other
Appl.
Host
Item no.
Amount 0.6ML
Available
CARD HyperOva Request
Type Bio-active substances
Clone
Specific against other
Appl.
Host
Item no.
Amount 5*0.6ML
Available

Items 1 to 25 of 78 total

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