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Anti-Growth-Associated Protein-43 (GAP-43) Antibody

Anti-Growth-Associated Protein-43 (GAP-43) Antibody

Item no.	GAP43
Manufacturer	Aves Labs
Amount	200 uL
Category	Immunohistochemistry Western Blot Immunocytochemistry Reagents & Chemicals Standards, Ladders & Markers DNA Ladders
Type	Marker
Format	Liquid
Applications	WB, IHC, ICC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Chicken
Isotype	IgY
Conjugate/Tag	Unconjugated
ECLASS 10.1	32160500
ECLASS 11.0	32160500
UNSPSC	12352202
Alias	Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46)
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Category	Cell Markers, Neuronal Markers
Manufacturer - Targets	Growth-Associated Protein-43
Country of Origin	United States
Storage Conditions	Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight	43 kDa
Concentration	2 mg/mL
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
Quality Control	Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:1000 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions	1:500-1:1000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed cells. 1:1000-1:5000 for western blotting. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Volume	Regular vials contain 200 µ, l of this antibody mixture.
Description	This antipeptide IgY antibody was generated in chickens against a C-terminal sequence shared between the mouse (NP_032109) and rat (NP_05889.1) gene products. It has been validated with human, mouse and rat tissues.
Manufacturer - Statement	We produce our Anti-Growth-Associated Protein-43 chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is IgY fraction.
Target Description	Growth-Associated Protein-43 is a 274 amino acid cytoplasmic protein found in neurons and various other embryonic cell types. This protein also goes by a number of alternative names, including neuromodulin and F1. In cultured neurons, GAP-43 antibodies selectively stain the growth cones of axons. As the substrate for various phosphorylation events, it is believed that GAP-43 plays a critical role in axonal growth, although its exact function is still unclear.
Antibody Registry ID (RRID)	AB_2313546
Clonality	Polyclonal
Dilution Range ICC	1:500-1:1000
Dilution Range IHC	1:500-1:1000
Dilution Range Western Blot	1:1000-1:2000
Immunogen	Synthetic peptide
Immunogen Species	Mouse
Manufacturer - Form	IgY Fraction
Expiration	12 months from receipt of product
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes	Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of mouse GAP-43 coupled to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 uL

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Delivery expected until 9/4/2025

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