Comparison

Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Item no. GFAP
Manufacturer Aves Labs
Amount 200 uL
Category
Type Neuron Cell Marker
Format Liquid
Applications WB, IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Conjugate/Tag Unconjugated
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41105337
Alias Glial fibrillary acidic protein, GFAP
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Cell Markers, Glial and Schwann Cell Markers
Manufacturer - Targets
Glial Fibrillary Acidic Protein (GFAP)
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight
50 kDa
Concentration
2 mg/mL (based on absorbance at 280 nm)
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
Quality Control
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Description
Our Anti-Glial Fibrillary Acidic Protein (GFAP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP), and is IgY fraction. It is great for use in IHC, ICC, WB.
Manufacturer - Statement
We produce our Anti-Glial Fibrillary Acidic Protein (GFAP) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is IgY fraction.
Target Description
Human Glial Fibrillary Acidic Protein (GFAP) is a 49, 749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
Antibody Registry ID (RRID)
AB_2313547
Clonality
Polyclonal
Dilution Range ICC
1:500-1:1000
Dilution Range IHC
1:500-1:1000
Dilution Range Western Blot
1:1000-1:2000
Immunogen
Recombinant human GFAP expressed in Escherichia coli
Immunogen Species
Human
Manufacturer - Form
IgY Fraction
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Antibodies were prepared by injecting laying hens first with highly purified recombinant human GFAP (produced in bacteria), followed by boosts of native GFAP protein purified from bovine spinal cords. After a series of boosts, eggs were collected from hyperimmunized animals and the IgY fraction prepared.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 uL
Available: In stock
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