Comparison

Anti-Prion Protein Antibody

Item no. PRN
Manufacturer Aves Labs
Amount 200 uL
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C, CD antigen CD230
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Cell Markers, Neuronal Markers
Manufacturer - Targets
Prion Protein (PRN)
Country of Origin
United States
Storage Conditions
Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.
Molecular Weight
27 kDa
Concentration
10 mg/mL
Shipping Method
Overnight Priority
Buffer
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.
Product Info
Prion protein is the protein responsible for human Creutzfeldt-Jakob Disease (CJD), and somatic mutations of the prion gene are responsible for Gerstmann-Straussler-Scheinker disease and Fatal Familial Insomnia. The bovine homolog of the prion protein is responsible for bovine Spongioform Encephalopathy (BSE), also known as “, Mad Cow Disease.”, In all cases, the prion protein has two folding states, with one state being the “, normal”, non-pathological form, and the other state inducing a spongioform encephalopathy.
Quality Control
Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.
Description
Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.
Manufacturer - Statement
We produce our Anti-Prion Protein (PRN) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is mixture of IgY fraction and affinity-purified antibodies.
Target Description
Prion protein is a ubiquitously-expressed, 27661 dalton protein (253 amino acids, NP_898902.1) of unclear function(s) in eukaryotes. This protein can exist in one of two conformational forms. One is its normally-folded form (PrPc or "cellular PrP"), which contains a mixture of alpha helical regions and a few beta-pleated sheets. The other is a misfolded form (PrPsc or "scrapie PrP") whose structure contains mostly beta-pleated sheets. This misfolded scrapie PrP form is cytotoxic, especially to nervous tissue, and can serve as a "seed, " inducing adjacent, normally-folded PrP proteins to misfold into the cytotoxic conformation. In this sense, the scrapie PrP can be thought of as an infectious agent both within the organism as well between organisms.
Antibody Registry ID (RRID)
AB_2313559
Clonality
Polyclonal
Dilution Range ICC
1:1000-1:2000
Dilution Range IHC
1:1000-1:2000
Dilution Range Western Blot
1:2500-1:5000
Immunogen
Recombinant fragment of human prion
Immunogen Species
Human
Manufacturer - Form
Mixture of IgY fraction and affinity-purified antibodies
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with a recombinant fragment of human PRN protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PRN protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 uL
Available: In stock
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