Gold-in-a-Box Conjugation Kit, 40 nm, 100 mL

The Gold-in-a-Box Conjugation Kit, 40 nm, contains the reagents required to prepare highly-reactive antibody or soluble protein gold conjugates that can be utilized in lateral-flow assays.
The key to most high-performance lateral-flow assays is the ability to covalently attach antibodies/proteins to gold nanoparticles. The Gold-in-a-Box Conjugation Kit enables optimal binding of an antibody/protein to our Naked Gold 40 nm highly concentrated gold sol, while retaining a high degree of specific activity through adjusting the pH of the gold sols to slightly above the isoelectric point of the coating antibody/protein. This is done through a series of pH titrations with the provided buffers. The Gold-in-a-Box kit allows researchers and assay developers to quickly (in less than 50 minutes) determine the pI and optimal coating conditions for your antibody or soluble protein and to perform the conjugation reaction. A sample preparation and gold conjugation procedure is provided.
Reagents are also provided to coat the resultant gold conjugates on polyester of glass fiber ribbon.

Kit Contents:

NG40-B100 – Naked Gold 40 nm Concentrated Gold Sol at 15 O.D./mL, 100 mL
BUFA-001 – Buffer Solution A, 1.0 mL
BUFB-001 – Buffer Solution B, 1.0 mL
BUFC-001 – Buffer Solution C, 1.0 mL
BUFD-001 – Buffer Solution D, 1.0 mL
BLK-005 – BSA Blocking Stabilizer Solution, 5.0 mL
CDB-005 – Gold Drying Buffer, 5.0 mL

7 x 4.5 x 3.5 in

Ambient temperatures

Victoria Martinez-Sernandez, Laura Muino, Maria Jesus Perteguer, Teresa Garate, Mercedes Mezo, Marta Gonzalez-Warleta, Antonio Muro, Jose Manuel Correia da Costa, Fernanda Romaris, Florencio M. Ubeira. Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis. 2011.

Giyoung Kim, Ji-Hea Moon, Saet Byeol Park, Youn-Jung Jang, Jongguk Lim, Changyeun Mo. Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7. Journal of Biosystems Engineering. 2013; 38(4): 335-340.

Sangdae Lee, Giyoung Kim and Jihea Moon. Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System. Sensors. 2013; 13(4): 5109-5116.