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Anti-Mouse MHC Class II (I-A/I-E) Purified

Anti-Mouse MHC Class II (I-A/I-E) Purified

Item no.	86212-20-100
Manufacturer	BioGems
Amount	100 ug
Category	Oncology Antibodies By Cell Type Carcinoma Melanoma Adenocarcinoma Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Primary Antibodies
Type	Antibody
Format	Purified
Applications	WB, FC, IP, IHC
Clone	M5/114.15.2
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b Kappa
Citations	Bhattacharya, A. L. O. K., Dorf, M. E., & Springer, T. A. (1981). A shared alloantigenic determinant on Ia antigens encoded by the IA and IE subregions: evidence for I region gene duplication. The Journal of Immunology, 127(6), 2488-2495. Mendiratta, S. K., Singh, N., Bal, V., & Rath, S. (1996). Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.Immunology, 89(2), 238-244. Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., & Mellman, I. (2007). The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proceedings of the National Academy of Sciences,104(1), 234-239.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Description	The M5/114.15.2 monoclonal antibody or (M5/114) is specific for the mouse major histocompatibility complex class II, specific for the I-A and I-E subregion-encoded glycoproteins (alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, but not the I-Af, I-Ak, nor I-As antigens). The antibody also reacts with cells from the H-2p or H-2r haplotypes mice. The polymorphic determinant represented by the mouse major histocompatibility complex class II (MHC Class II) is expressed by the immune cells bearing the specified alloantigens, like T and B lymphocytes, monocytes, dendritic cells, and macrophages. The antibody doesn’t react with the NOD H-2g7 haplotype alloantigens. M5/114 mAb inhibits I-A-restricted T cell responses of the Haplotypes H-2b, H-2d, H-2q, and H-2u.
Storage Temperature	4C
Formulation	Phosphate-buffered aqueous solution, ≤ 0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Preperation	The product should be stored undiluted at 4C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Interest	Innate Immunity, Adaptive Immunity
Format	Purified

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

86212-20-100-datasheet.pdf

86212-20-100-safety-datasheet.pdf