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Anti-Mouse TCR beta BG Violet 450

Anti-Mouse TCR beta BG Violet 450

Item no.	89612-40-25
Manufacturer	BioGems
Amount	25 ug
Category	Antibodies Cell Biology Cell Status Necrosis Flow Cytometry Primary Antibodies
Type	Antibody
Format	BG Violet 450
Applications	FC
Clone	H57-597
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Isotype	IgG
Conjugate/Tag	BG Violet 450
Citations	Kubo, R. T., Born, W., Kappler, J. W., Marrack, P., & Pigeon, M. (1989). Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. The Journal of Immunology, 142(8), 2736-2742. Gascoigne, N. R. (1990). Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. Journal of Biological Chemistry, 265(16), 9296-9301. Kruisbeek, A. M., Shevach, E., & Thornton, A. M. (2004). Proliferative assays for T cell function. Current Protocols in Immunology, 3-12. Saint-Ruf, C., Panigada, M., Azogui, O., Debey, P., von Boehmer, H., & Grassi, F. (2000). Different initiation of pre-TCR and γδTCR signalling. Nature,406(6795), 524-527.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Description	The H57-597 monoclonal antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The crosslinking induces activation and proliferation of T cells, as a plate-bound or a soluble H57-597, or the plate-bound antibody can induce apoptosis, based on assay conditions. The beta chain of the TCR can combine with the alpha chain of the receptor to produce the alpha-beta TCR, which is expressed by the NKT cells, by the NK1.1+ thymocytes and most of the T cells. The beta chain does not react with the gamma-delta TCR-bearing cells, expressed by a small number of T cells. This antibody can be used as a phenotypic marker for the TCR beta expressing cells or for the functional purpose of TCR-mediated cell activation or apoptosis. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
Storage Temperature	4C
Formulation	Phosphate-buffered aqueous solution, ≤ 0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Preperation	The product should be stored undiluted at 4C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Interest	Adaptive Immunity
Manufacturers conjugate	BG Violet 450

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

89612-40-25-datasheet.pdf

89612-40-25-safety-datasheet.pdf