Comparison

CFSE 500 ug

Manufacturer BioGems
Category
Type Solution
Specific against other
Applications FC
Amount 500 ug
Item no. 63310-00-500
eClass 6.1 32120508
eClass 9.0 32120508
Available
Description
CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin
Formulation
Lyophilized
References

Ingulli, E. (2007). Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells. In Immunological Tolerance (pp. 365-376). Humana Press.

Lyons, A. B., & Parish, C. R. (1994). Determination of lymphocyte division by flow cytometry. Journal of immunological methods, 171(1), 131-137.

Parish, C. R., Glidden, M. H., Quah, B. J., & Warren, H. S. (2009). Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current protocols in immunology, 4-9.

Application Notes
CFSE has been tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. It can be used at concentrations ranging from 0.5 to 20 uM for cell labeling. For optimal performance, it is recommended that the used concentration be empirically determined for the assay of interest.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
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