Manufacturer |
Biomatik
|
Category |
|
Type |
Clia |
Specific against |
Human |
Format |
96 well plate |
Applications |
CLIA |
Amount |
96T |
Item no. |
BM-EKU08960 |
eClass 6.1 |
27150207 |
eClass 9.0 |
27150207 |
Available |
|
Detection Range |
468.75-30000 pg/mL |
Sensitivity |
17.4 pg/mL |
Sample Type |
Tissue homogenates, cell lysates and other biological fluids |
Assay Time |
2 hours, 40min |
Gene Name |
Nucleotide Binding Oligomerization Domain Containing Protein 2 |
Gene Aliases |
CD; ACUG; BLAU; CARD15; CLR16.3; IBD1; NLRC2; NOD2B; PSORAS1; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1 |
Assay Type |
Sandwich |
Detection Method |
Chemiluminescent |
Shipping Condition |
Blue ice |
Storage Instructions |
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
Precaution of Use |
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
Expiry Date |
8 months |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). No significant cross-reactivity or interference between Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) and analogues was observed. |
Test Principle |
The microplate provided in this kit has been pre-coated with an antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) level in the sample or standard.; |
Assay Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Restrictions |
For research use only. Not for diagnostic procedures. |
Quality Systems |
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
Research Area |
Signal transduction; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.