« Back
Home /
Human Amphiregulin (AREG) ELISA Kit (CLIA)

Human Amphiregulin (AREG) ELISA Kit (CLIA)

Manufacturer	Biomatik
Category	Oncology By Cell Type ELISA & Immunoassays Immunoassay CLIA Kits CLIA
Type	Clia
Specific against	Human
Format	96 well plate
Applications	CLIA
Amount	96T
Item no.	BM-EKU09428
Targets	AREG
eClass 6.1	27150207
eClass 9.0	27150207
Available
Detection Range	15.62-1000 pg/mL
Sensitivity	0.49 pg/mL
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time	2 hours, 40min
UniProt ID	P15514
Gene ID	374
Gene Name	Amphiregulin
Gene Aliases	AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
Assay Type	Sandwich
Detection Method	Chemiluminescent
Shipping Condition	Blue ice
Storage Instructions	-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use	Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Amphiregulin (AREG). No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.
Test Principle	The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.;
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Cytokine; Tumor immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 96T

available

Delivery expected until 5/30/2024