Mouse monoclonal antibody to human p75NTR [ME20.4]: IgG

Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF, 113505), neurotrophin-3 (NTF3, 162660), and neurotrophin-4/5 (NTF5, 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB, see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1, 191315), TRKB (NTRK2, 600456), and TRKC (NTRK3, 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity

It is recommended that a thawed sample is stored at 4AC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20AC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.

Lyophilised

Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.

This antibody recognises p75NTR (low affinity neurotrophin receptor)

1. Liu W. et al (2012) Distribution of P75 neurotrophin receptor in adult human cochlea-an immunohistochemical study. Cell Tissue Res. 2012 Mar 31.
2. Inoue K. et al. (2009) Differential expression of stem-cell-associated markers in human hair follicle epithelial cells. Lab Invest. 2009 Aug, 89(8):844-56.
3. Ariga M. et al. (2008) Functional role of sortilin in myogenesis and development of insulin-responsive glucose transport system in C2C12 myocytes J Biol Chem. 2008 Apr 11, 283(15):10208-20
4. Rogers ML et al (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010 Sep 14, 226(1-2):93-103.