Rabbit polyclonal antibody to human Gremlin-1 (165-184): Affinity purified

Gremlin-1 is a secreted cytokine that may play a role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. It is highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Gremlin-1 is induced through TGF-beta 1-mediated pathways in Mesangial cell and it is down-regulated in tumor cell lines.

At least 12 months after purchase at 2 - 4AC (lyophilized formulations).After reconstitution, aliquot and store at -20AC for a higher stability and at 4AC with an appropriate antibacterial agent.Avoid freeze-thaw cycles

A synthetic peptide (TKKKRVTRVKQCRCISIDLD) corresponding to a region (165-184 aa) from the C-terminus of human Gremlin-1.

Rabbit

Reconstitute in 100 Aul of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.