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Mouse monoclonal antibody to Nestin [4D11] (317-630)

Mouse monoclonal antibody to Nestin [4D11] (317-630)

Item no.	M-1385-100
Manufacturer	Biosensis
Amount	100ul
Category	Neuroscience Molecular Targets for Neuroscience Neural Development Antibodies Western Blot Immunocytochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, ICC
Clone	4D11
Specific against	other
Host	Mouse
Isotype	IgG
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Similar products	Nestin, NES
Available
Sub category	Development-Stem Cells-Cancer-Cell Markers-Neuronal Markers
products_extra_fields_value	Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240kDa. However the real molecular weight is considerably less than this, at 177kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
products_quantity	199
storage	After reconstitution of lyophilised antibody, aliquot and store at -20AC for a higher stability. Avoid freeze-thaw cycles.
formulation	Lyophilised with 5% trehalose
immunogen	Partial segment (region 317-630 aa) of human Nestin expressed in E.coli
crossreactivity	Human, Rodent
conality	Mouse
specificity	This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band.
reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ul

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Delivery expected until 11/13/2025

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