Mouse monoclonal antibody to Tyrosine Hydroxylase clone (LNC 1)

Tyrosine hydroxylase is an excellent marker for dopaminergic and noradrenergic neurons. Tyrosine hydroxylase (a.k.a. tyrosine 3-monooxygenase)A is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3, 4-dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs). http://en.wikipedia.org/wiki/Tyrosine_hydroxylase

After reconstitution keep aliquots at -20AC to -70C for a higher stability. At 4AC keep up to one week, insulated, protected from light, use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light

Lyophilized, dry powder.

Chicken, Frog, Horse, Human, Monkey, Mouse, Vole, Sheep, Zebrafish other species not yet tested

Clone LNC 1 recognizes an epitope on the outside of the regulatory N-terminus. The clone detects a protein of approximately 59-61 kDa by Western blot and reduced SDS-PAGE. The clone does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT) by western blots.

Kapatos G., Kemski V., and Geddes T. (1989) Dopamine neurons in monolayer culture as a model system for the study of tyrosine hydroxylase, in Pteridines and Biogenic Amines in Neuropsychiatry, Pediatrics and Immunology (Levine R. A., KuhnD. M., Milstien S., and Curtius H-C., eds), pp. 243a€”258. Lakeshore Publishers, Grosse Pointe, Michigan.