Human TNF-beta ELISA Kit (2 plates)

The human TNF-beta Kit is a sandwich ELISA. The capture antibody is a polyclonal human TNF-beta antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-beta concentration are added to these wells and allowed to complex with the bound TNF-beta antibody. A biotinylated human TNF-beta polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-beta present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-beta in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

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