Human Interleukin 1 receptor antagonist ELISA Kit (2 plates)

The human Interleukin 1 receptor antagonist (IL-1RN) Kit is a sandwich ELISA. The capture antibody is a polyclonal human IL-1RN antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-1RN concentration are added to these wells and allowed to complex with the bound IL-1RN antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1RN present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-1RN in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

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