Human Nerve Growth Factor Receptor-Fc Chimera (222 amino acids) expressed in mammalian cells

Nerve growth factor (NGF) receptor, also known as p75NTR, A is a low affinity NGF receptor. It binds with equal affinity neurotrophins such as beta NGF, BDNF, NT-3 and NT-4. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The NGF receptor is a type I transmembrane glycoprotein (399 aa) consisting of a signal peptide (28 aa), an extracellular domain (222 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 aa) and a cytoplasmic domain (155 aa).

Lyophilized products should be stored at 2 to 8AC. Following reconstitution short-term storage at 4AC is recommended, and longer-term storage of aliquots at -18 to -20AC. Repeated freeze thawing is not recommended.

The NGF Receptor-Fc chimera consists of 30-50% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.

It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.