Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®568 Tyramide

Blue ice

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure.    CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Red fluorescent CF® 543 has excitation/emission at 562/583 nm.    Kit Components: 

 
• CF® 568 tyramide stock solution
 
• HRP Goat anti-rabbit IgG
 
• BSA for blocking
 
• Amplification buffer
 
• Hydrogen peroxide 30% stablized solution
 

    We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

IgG from domesticated goat serum (Capra aegagrus hircus); BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana)

6 months

2014

II

Danger

Hydrogen peroxide 30%, CAS 7722-84-1 (component 99823-100uL); Dimethylsulfoxide >99%, CAS 67-68-5 (tyramide component)

6 months