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AccuEasy Flow Cytometry Kit

AccuEasy Flow Cytometry Kit

Item no.	B-30069
Manufacturer	Biotium
Amount	1 kit
Category	Oncology Food Safety Allergenes Neuroscience Aging & Neurological Disorders Other Assays & Kits Other Kits By Organ Lung Cancer Breast Cancer
Type	Kit
Specific against	other
Citations	Endo, H.,Yano, M.,Okumura, Y., and Kido, H. (2014). Ibuprofen enhances the anticancer activity of cisplatin in lung cancer cells by inhibiting the heat shock protein 70. Cell Death Dis 5, e1027. 10.1038/cddis.2013.550 (CF488A Annexin V; FC) Gautam, N.,Roy, U.,Balkundi, S.,Puligujja, P.,Guo, D.,Smith, N.,Liu, X. M.,Lamberty, B.,Morsey, B.,Fox, H. S., et al. (2013). Preclinical Pharmacokinetics and Tissue Distribution of Long-Acting Nanoformulated Antiretroviral Therapy. Antimicrob Agents Chemother. DOI:10.1128/AAC.00267-13 (CF633 succinimidyl ester; nanoparticles; IF; FC) Gielen, P. R.,Aftab, Q.,Ma, N.,Chen, V. C.,Hong, X.,Lozinsky, S.,Naus, C. C., and Sin, W. C. (2013). Connexin43 confers Temozolomide resistance in human glioma cells by modulating the mitochondrial apoptosis pathway. Neuropharmacology. DOI:10.1016/j.neuropharm.2013.05.002 (CF405M Annexin V; FC) Giuntini, S.,Reason, D. C., and Granoff, D. M. (2012). Combined roles of human IgG subclass, alternative complement pathway activation, and epitope density in the bactericidal activity of antibodies to meningococcal factor h binding protein. Infect Immun 80, 187-194. DOI:10.1128/IAI.05956-11 (CF488A goat anti-human IgG; FC) Kesik, M, Demir, B, Barlas, FB, Geyik, C, Cevher, SC, Odaci Demirkol, D, Timur, S, Cirpan, A, and Toppare, L. (2015). A multi-functional fluorescent scaffold as a multi-colour probe: design and application in targeted cell imaging. RSC Advances 5: 83361-83367. (Mix-n-Stain CF555; FC) Li, X.,Saeki, R.,Watari, A.,Yagi, K., and Kondoh, M. (2014). Tissue distribution and safety evaluation of a claudin-targeting molecule, the C-terminal fragment of Clostridium perfringens enterotoxin. Eur J Pharm Sci 52, 132-137. 10.1016/j.ejps.2013.10.018 (Xenolight CF750; FC, in vivo imaging) Mahassni, S. H., and Al-Reemi, R. M. (2013). Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (Lepidium sativum) seeds. Saudi Journal of Biological Sciences 20, 131-139. DOI: dx.doi.org/10.1016/j.sjbs.2012.12.002 (CF488A Annexin V; IF, FC) Mikosik, A.,Foerster, J.,Jasiulewicz, A.,Frackowiak, J.,Colonna-Romano, G.,Bulati, M.,Buffa, S.,Martorana, A.,Caruso, C.,Bryl, E., et al. (2013). Expression of calpain-calpastatin system (CCS) member proteins in human lymphocytes of young and elderly individuals; pilot baseline data for the CALPACENT project. Immun Ageing 10, 27. DOI:10.1186/1742-4933-10-27 (Mix-n-Stain CF555; FC) Osteikoetxea, X, Balogh, A, Szabo-Taylor, K, Nemeth, A, Szabo, TG, Paloczi, K, Sodar, B, Kittel, A, György, B, Pallinger, E, et al. (2015). Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties. PLoS One 10: e0121184. (Mix-n-Stain CF488A; FC) Ruger, M.,Ackermann, M., and Reichl, U. (2014). Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry. BMC Microbiol 14, 56. 10.1186/1471-2180-14-56 (CF405S Mix-n-Stain; FC) Ruger, M.,Bensch, G.,Tungler, R., and Reichl, U. (2012). A flow cytometric method for viability assessment of Staphylococcus aureus and Burkholderia cepacia in mixed culture. Cytometry A. DOI:10.1002/cyto.a.22219 (CF405S Mix-n-Stain labeling of wheat germ agglutinin; FC) Sainski, A. M.,Dai, H.,Natesampillai, S.,Pang, Y. P.,Bren, G. D.,Cummins, N. W.,Correia, C.,Meng, X. W.,Tarara, J. E.,Ramirez-Alvarado, M., et al. (2014). Casp8p41 generated by HIV protease kills CD4 T cells through direct Bak activation. J Cell Biol 207, 159. 10.1083/jcb.20140505109252014c (CF640R Mix-n-Stain; FC) Skindersoe, M. E., Rohde, M., and Kjaerulff, S. (2012). A novel and rapid apoptosis assay based on thiol redox status. Cytometry A. DOI:10.1002/cyto.a.22032 (CF647 Annexin V; IF, FC) Wienand, K., and Shires, K. (2015). The Use of MAGE C1 and Flow Cytometry to Determine the Malignant Cell Type in Multiple Myeloma. PLoS ONE 10(3): e0120734. doi:10.1371/journal.pone.0120734 (CF488A Mix-n-Stain; FC) Zeiderman, M. R.,Egger, M. E.,Kimbrough, C. W.,England, C. G.,Dupre, T. V.,McMasters, K. M., and McNally, L. R. (2014). Targeting of BRAF resistant melanoma via extracellular matrix metalloproteinase inducer receptor. J Surg Res 190, 111-118. 10.1016/j.jss.2014.02.021 (CF750 succinimidyl ester; in vivo imaging, FC)
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Description	The conventional flow cytometry method for analyzing cell surface markers on adherent cells requires detachment of cells from their culture surface before subjecting to antibody staining. Unfortunately, cell detachment introduces a significant stress to cells which can lead to changes in expression of cell surface markers. In addition, multiple centrifugation step often are required during the antibody staining, which is not only laborious, but also can lead to cell loss. The AccuEasy™ flow cytometry kit offered by Biotium providing you a much simpler, more accurate and often more sensitive method for detecting cell surface markers on adherent cells than the conventional method. The AccuEasy™ method eliminates laborious centrifugation steps. More importantly, AccuEasy™ allows you to detect cell surface marker expression on cells in their native adherent state without perturbation caused by cell detachment. The AccuEasy™ method can yield substantially higher fluorescence signal for cell surface markers compared to conventional methods. See our Flow Cytometry Brochure for more products for flow cytometry.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 kit

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Delivery expected until 9/25/2025

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