Hoechst 33258, 10mg/mL in H2O

Fluorescence Microscopy|Nuclear Counterstains|Super-Resolution Imaging|Cell & Organelle Stains>Nuclear Stains &; DNA/RNA Dyes|Microbiology>Other Bacterial Stains|PCR &; DNA Amplification>DNA Binding Dyes

For ease of use and safer handling, we offer both Hoechst 33258 and Hoechst 33342 dissolved in H₂O at 10 mg/mL. Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains. Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required. 

 
• &lamba; _Ex/&lamba; _Em (with DNA) = 352/461 nm
 
• Light yellow solution
 
• Store at 4°C and protect from light
 
• C₂₅H₂₇Cl₃N₆O
 
• MW: 533.88
 

   

Protect from light;

Stable for at least 1 month

Warning

Cytology reagents or solutions or stains

H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.