Annexin V, CF®450 Conjugate

Blue ice

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF® Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF® 450 dye has excitation/emission at 450/538 nm.

BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells, Annexin V (recombinant produced in E. coli)

6 months

41116113

Sodium azide, CAS 26628-22-8, <0.1%

Sodium azide, CAS 26628-22-8, <0.1%