Anti-mTOR (S2442) Antibody

Primary Antibodies

288892 MW

WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:50-1:200

Serine/threonine-protein kinase mTOR

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Serine/threonine protein kinase which is a central
regulator of cellular metabolism, growth and survival in response
to hormones, growth factors, nutrients, energy and stress signals.
MTOR directly or indirectly regulates the phosphorylation of at
least 800 proteins. Functions as part of 2 structurally and
functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR
complex 1 and 2). Activated mTORC1 up-regulates protein synthesis
by phosphorylating key regulators of mRNA translation and ribosome
synthesis. This includes phosphorylation of EIF4EBP1 and release
of its inhibition toward the elongation initiation factor 4E
(eiF4E). Moreover, phosphorylates and activates RPS6KB1 and
RPS6KB2 that promote protein synthesis by modulating the activity
of their downstream targets including ribosomal protein S6,
eukaryotic translation initiation factor EIF4B, and the inhibitor
of translation initiation PDCD4. Stimulates the pyrimidine
biosynthesis pathway, both by acute regulation through RPS6KB1-
mediated phosphorylation of the biosynthetic enzyme CAD, and
delayed regulation, through transcriptional enhancement of the
pentose phosphate pathway which produces 5-phosphoribosyl-1-
pyrophosphate (PRPP), an allosteric activator of CAD at a later
step in synthesis, this function is dependent on the mTORC1
complex. Regulates ribosome synthesis by activating RNA polymerase
III-dependent transcription through phosphorylation and inhibition
of MAF1 an RNA polymerase III-repressor. In parallel to protein
synthesis, also regulates lipid synthesis through SREBF1/SREBP1
and LPIN1. To maintain energy homeostasis mTORC1 may also regulate
mitochondrial biogenesis through regulation of PPARGC1A. mTORC1
also negatively regulates autophagy through phosphorylation of
ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-
758', disrupting the interaction with AMPK and preventing
activation of ULK1. Also prevents autophagy through
phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a
feedback control on upstream growth factor signaling that includes
phosphorylation and activation of GRB10 a INSR-dependent signaling
suppressor. Among other potential targets mTORC1 may phosphorylate
CLIP1 and regulate microtubules. As part of the mTORC2 complex
MTOR may regulate other cellular processes including survival and
organization of the cytoskeleton. Plays a critical role in the
phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of
phosphoinositide 3-kinase, facilitating its activation by PDK1.
mTORC2 may regulate the actin cytoskeleton, through
phosphorylation of PRKCA, PXN and activation of the Rho-type
guanine nucleotide exchange factors RHOA and RAC1A or RAC1B.
mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'
(PubMed:12087098, PubMed:12150925, PubMed:12150926,
PubMed:12231510, PubMed:12718876, PubMed:14651849,
PubMed:15268862, PubMed:15467718, PubMed:15545625,
PubMed:15718470, PubMed:18497260, PubMed:18762023,
PubMed:18925875, PubMed:20516213, PubMed:20537536,
PubMed:21659604, PubMed:23429703, PubMed:23429704,
PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by
adjusting the expression of CEBPB isoforms (By similarity).

Boster Bio Anti-mTOR (S2442) Antibody catalog # A00003S2442. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Serine/threonine-protein kinase mTOR, 2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1