Anti-Daxx (phospho-S668) Antibody

Primary Antibodies

81373 MW

WB: 1:500-1:1000

Death domain-associated protein 6

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Transcription corepressor known to repress
transcriptional potential of several sumoylated transcription
factors. Down-regulates basal and activated transcription. Its
transcription repressor activity is modulated by recruiting it to
subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear
bodies through interactions with MCSR1 and PML, respectively.
Seems to regulate transcription in PML/POD/ND10 nuclear bodies
together with PML and may influence TNFRSF6-dependent apoptosis
thereby. Inhibits transcriptional activation of PAX3 and ETS1
through direct protein-protein interactions. Modulates PAX5
activity; the function seems to involve CREBBP. Acts as an adapter
protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger
E3 ligase MDM2 ubiquitination activity. Under non-stress
condition, in association with the deubiquitinating USP7, prevents
MDM2 self-ubiquitination and enhances the intrinsic E3 ligase
activity of MDM2 towards TP53, thereby promoting TP53
ubiquitination and subsequent proteasomal degradation. Upon DNA
damage, its association with MDM2 and USP7 is disrupted, resulting
in increased MDM2 autoubiquitination and consequently, MDM2
degradation, which leads to TP53 stabilization. Acts as histone
chaperone that facilitates deposition of histone H3.3. Acts as
targeting component of the chromatin remodeling complex ATRX:DAXX
which has ATP-dependent DNA translocase activity and catalyzes the
replication-independent deposition of histone H3.3 in pericentric
DNA repeats outside S-phase and telomeres, and the in vitro
remodeling of H3.3-containing nucleosomes. Does not affect the
ATPase activity of ATRX but alleviates its transcription
repression activity. Upon neuronal activation associates with
regulatory elements of selected immediate early genes where it
promotes deposition of histone H3.3 which may be linked to
transcriptional induction of these genes. Required for the
recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-
NBs); the process is independent of ATRX and facilitated by ASF1A;
PML-NBs are suggested to function as regulatory sites for the
incorporation of newly synthesized histone H3.3 into chromatin. In
case of overexpression of centromeric histone variant CENPA (as
found in various tumors) is involved in its mislocalization to
chromosomes; the ectopic localization involves a heterotypic
tetramer containing CENPA, and histones H3.3 and H4 and decreases
binding of CTCF to chromatin. Proposed to mediate activation of
the JNK pathway and apoptosis via MAP3K5 in response to signaling
from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent
interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated
apoptosis. In contrast, in lymphoid cells JNC activation and
TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction
activity towards human cytomegalovirus (HCMV). Plays a role as a
positive regulator of the heat shock transcription factor HSF1
activity during the stress protein response (PubMed:15016915).

Boster Bio Anti-Daxx (phospho-S668) Antibody catalog # A00439S668. Tested in WB applications. This antibody reacts with Human.