Anti-KEAP1 Antibody

Primary Antibodies

69666 MW

WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Kelch-like ECH-associated protein 1

KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.

KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

Kelch-like ECH-associated protein 1

Cytoplasm. Nucleus. Shuttles between
cytoplasm and nucleus.

Broadly expressed, with highest levels in
skeletal muscle.

At least two isoforms of KEAP1 are known to exist.