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Anti-Axin-1 AXIN1 Antibody

Anti-Axin-1 AXIN1 Antibody

Item no.	BOS-A00986
Manufacturer	Boster
Amount	0.1 mg
Category	Oncology By Cell Type Carcinoma Cell Biology Cell Status Apoptosis Western Blot ELISA Immunofluorescence Immunostaining
Format	Liquid
Applications	WB, IF, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	AXIN,PPP1R49,AXIN,Axin-1,Axis inhibition protein 1,hAxin
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	AXIN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	95635 MW
Clonality	Polyclonal
Application Details	AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	AXIN1
Gene Full Name	Axin 1
Background	AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
Immunogen	AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1.
Contents	AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	AXIN1 Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Signal Transduction
Protein Name	Axin 1
Protein Function	Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684).
Subcellular Localization	Cytoplasm
Description	Boster Bio Anti-Axin-1 AXIN1 Antibody (Catalog # A00986). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Ubiquitously expressed.
Predicted Reactivity	Chicken

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A00986-datasheet.pdf

BOS-A00986-safety-datasheet.pdf