Anti-Phospho-PKC epsilon (S729) PRKCE Antibody

Primary Antibodies

83674 MW

WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000

Protein kinase C epsilon type

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Calcium-independent, phospholipid- and diacylglycerol
(DAG)-dependent serine/threonine-protein kinase that plays
essential roles in the regulation of multiple cellular processes
linked to cytoskeletal proteins, such as cell adhesion, motility,
migration and cell cycle, functions in neuron growth and ion
channel regulation, and is involved in immune response, cancer
cell invasion and regulation of apoptosis. Mediates cell adhesion
to the extracellular matrix via integrin-dependent signaling, by
mediating angiotensin-2-induced activation of integrin beta-1
(ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which
phosphorylates and activates PTK2/FAK, leading to the spread of
cardiomyocytes. Involved in the control of the directional
transport of ITGB1 in mesenchymal cells by phosphorylating
vimentin (VIM), an intermediate filament (IF) protein. In
epithelial cells, associates with and phosphorylates keratin-8
(KRT8), which induces targeting of desmoplakin at desmosomes and
regulates cell-cell contact. Phosphorylates IQGAP1, which binds to
CDC42, mediating epithelial cell-cell detachment prior to
migration. In HeLa cells, contributes to hepatocyte growth factor
(HGF)-induced cell migration, and in human corneal epithelial
cells, plays a critical role in wound healing after activation by
HGF. During cytokinesis, forms a complex with YWHAB, which is
crucial for daughter cell separation, and facilitates abscission
by a mechanism which may implicate the regulation of RHOA. In
cardiac myocytes, regulates myofilament function and excitation
coupling at the Z-lines, where it is indirectly associated with F-
actin via interaction with COPB1. During endothelin-induced
cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which
is critical for cardiomyocyte survival and regulation of sarcomere
length. Plays a role in the pathogenesis of dilated cardiomyopathy
via persistent phosphorylation of troponin I (TNNI3). Involved in
nerve growth factor (NFG)-induced neurite outgrowth and neuron
morphological change independently of its kinase activity, by
inhibition of RHOA pathway, activation of CDC42 and cytoskeletal
rearrangement. May be involved in presynaptic facilitation by
mediating phorbol ester-induced synaptic potentiation.
Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2
(GABRG2), which reduces the response of GABA receptors to ethanol
and benzodiazepines and may mediate acute tolerance to the
intoxicating effects of ethanol. Upon PMA treatment,
phosphorylates the capsaicin- and heat-activated cation channel
TRPV1, which is required for bradykinin-induced sensitization of
the heat response in nociceptive neurons. Is able to form a
complex with PDLIM5 and N-type calcium channel, and may enhance
channel activities and potentiates fast synaptic transmission by
phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2).
In prostate cancer cells, interacts with and phosphorylates STAT3,
which increases DNA-binding and transcriptional activity of STAT3
and seems to be essential for prostate cancer cell invasion.
Downstream of TLR4, plays an important role in the
lipopolysaccharide (LPS)-induced immune response by
phosphorylating and activating TICAM2/TRAM, which in turn
activates the transcription factor IRF3 and subsequent cytokines
production. In differentiating erythroid progenitors, is regulated
by EPO and controls the protection against the TNFSF10/TRAIL-
mediated apoptosis, via BCL2. May be involved in the regulation of
the insulin-induced phosphorylation and activation of AKT1.

Boster Bio Anti-Phospho-PKC epsilon (S729) PRKCE Antibody catalog # A01151S729-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.