Anti-LIS1 PAFAH1B1 Antibody

Primary Antibodies

46638 MW

LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1.

LIS1 Antibody is affinity chromatography purified via peptide column.

Neuroscience

Required for proper activation of Rho GTPases and actin
polymerization at the leading edge of locomoting cerebellar
neurons and postmigratory hippocampal neurons in response to
calcium influx triggered via NMDA receptors. Non-catalytic subunit
of an acetylhydrolase complex which inactivates platelet-
activating factor (PAF) by removing the acetyl group at the SN-2
position (By similarity). Positively regulates the activity of the
minus-end directed microtubule motor protein dynein. May enhance
dynein-mediated microtubule sliding by targeting dynein to the
microtubule plus end. Required for several dynein- and
microtubule-dependent processes such as the maintenance of Golgi
integrity, the peripheral transport of microtubule fragments and
the coupling of the nucleus and centrosome. Required during brain
development for the proliferation of neuronal precursors and the
migration of newly formed neurons from the
ventricular/subventricular zone toward the cortical plate.
Neuronal migration involves a process called nucleokinesis,
whereby migrating cells extend an anterior process into which the
nucleus subsequently translocates. During nucleokinesis dynein at
the nuclear surface may translocate the nucleus towards the
centrosome by exerting force on centrosomal microtubules. May also
play a role in other forms of cell locomotion including the
migration of fibroblasts during wound healing. Required for dynein
recruitment to microtubule plus ends and BICD2-bound cargos
(PubMed:22956769).

Boster Bio Anti-LIS1 PAFAH1B1 Antibody (Catalog # A01273). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Pig, Bovine, Chicken