Anti-HSP60 (T547) HSPD1 Antibody

Primary Antibodies

61055 MW

WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200

60 kDa heat shock protein, mitochondrial

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Chaperonin implicated in mitochondrial protein import
and macromolecular assembly. Together with Hsp10, facilitates the
correct folding of imported proteins. May also prevent misfolding
and promote the refolding and proper assembly of unfolded
polypeptides generated under stress conditions in the
mitochondrial matrix (PubMed:1346131, PubMed:11422376). The
functional units of these chaperonins consist of heptameric rings
of the large subunit Hsp60, which function as a back-to-back
double ring. In a cyclic reaction, Hsp60 ring complexes bind one
unfolded substrate protein per ring, followed by the binding of
ATP and association with 2 heptameric rings of the co-chaperonin
Hsp10. This leads to sequestration of the substrate protein in the
inner cavity of Hsp60 where, for a certain period of time, it can
fold undisturbed by other cell components. Synchronous hydrolysis
of ATP in all Hsp60 subunits results in the dissociation of the
chaperonin rings and the release of ADP and the folded substrate
protein (Probable).

Boster Bio Anti-HSP60 (T547) HSPD1 Antibody catalog # A01280T547. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.