Anti-PKC Theta (G532) PRKCQ Antibody

Primary Antibodies

81865 MW

WB: 1:500-1:1000
IHC: 1:50-1:200

Protein kinase C theta type

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Calcium-independent, phospholipid- and diacylglycerol
(DAG)-dependent serine/threonine-protein kinase that mediates non-
redundant functions in T-cell receptor (TCR) signaling, including
T-cells activation, proliferation, differentiation and survival,
by mediating activation of multiple transcription factors such as
NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated
T-cells, is required for the activation of NF-kappa-B and JUN,
which in turn are essential for IL2 production, and participates
in the calcium-dependent NFATC1 and NFATC2 transactivation.
Mediates the activation of the canonical NF-kappa-B pathway
(NFKB1) by direct phosphorylation of CARD11 on several serine
residues, inducing CARD11 association with lipid rafts and
recruitment of the BCL10-MALT1 complex, which then activates IKK
complex, resulting in nuclear translocation and activation of
NFKB1. May also play an indirect role in activation of the non-
canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway
leading to JUN activation, acts by phosphorylating the mediator
STK39/SPAK and may not act through MAP kinases signaling. Plays a
critical role in TCR/CD28-induced NFATC1 and NFATC2
transactivation by participating in the regulation of reduced
inositol 1, 4, 5-trisphosphate generation and intracellular calcium
mobilization. After costimulation of T-cells through CD28 can
phosphorylate CBLB and is required for the ubiquitination and
subsequent degradation of CBLB, which is a prerequisite for the
activation of TCR. During T-cells differentiation, plays an
important role in the development of T-helper 2 (Th2) cells
following immune and inflammatory responses, and, in the
development of inflammatory autoimmune diseases, is necessary for
the activation of IL17-producing Th17 cells. May play a minor role
in Th1 response. Upon TCR stimulation, mediates T-cell protective
survival signal by phosphorylating BAD, thus protecting T-cells
from BAD-induced apoptosis, and by up-regulating BCL-X (L)/BCL2L1
levels through NF-kappa-B and JUN pathways. In platelets,
regulates signal transduction downstream of the ITGA2B, CD36/GP4,
F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in
'outside-in' signaling and granule secretion signal transduction.
May relay signals from the activated ITGA2B receptor by regulating
the uncoupling of WASP and WIPF1, thereby permitting the
regulation of actin filament nucleation and branching activity of
the Arp2/3 complex. May mediate inhibitory effects of free fatty
acids on insulin signaling by phosphorylating IRS1, which in turn
blocks IRS1 tyrosine phosphorylation and downstream activation of
the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence
of phosphatidylglycerol or phosphatidylinositol. Phosphorylates
PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its
ability to phosphorylate PKB/AKT1.

Boster Bio Anti-PKC Theta (G532) PRKCQ Antibody catalog # A01293. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.