Anti-Raptor Rptor Antibody

Primary Antibodies

149471 MW

Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 ?g/mL. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Regulatory associated protein of MTOR, complex 1

Raptor polyclonal antibody was raised against a 13 amino acid synthetic peptide from near the amino-terminus of human Raptor. The immunogen is located within amino acids 90 - 140 of Raptor.

Raptor Antibody is affinity chromatography purified via peptide column.

Signal Transduction, Neuroscience

Involved in the control of the mammalian target of
rapamycin complex 1 (mTORC1) activity which regulates cell growth
and survival, and autophagy in response to nutrient and hormonal
signals; functions as a scaffold for recruiting mTORC1 substrates.
mTORC1 is activated in response to growth factors or amino acids.
Growth factor-stimulated mTORC1 activation involves a AKT1-
mediated phosphorylation of TSC1-TSC2, which leads to the
activation of the RHEB GTPase that potently activates the protein
kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires
its relocalization to the lysosomes mediated by the Ragulator
complex and the Rag GTPases. Activated mTORC1 up-regulates protein
synthesis by phosphorylating key regulators of mRNA translation
and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and
releases it from inhibiting the elongation initiation factor 4E
(eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389',
which then promotes protein synthesis by phosphorylating PDCD4 and
targeting it for degradation. Involved in ciliogenesis.

Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Isoform 1, isoform 5 and isoform 7 are
expressed in a wide variety of tissues. Highest expression in
peripheral blood leukocytes, spleen, thymus and liver. Barely
detectable in brain, testis and skeletal muscle.