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Anti-AIF-M1 (Y85) Antibody

Anti-AIF-M1 (Y85) Antibody

Item no.	BOS-A01571Y85
Manufacturer	Boster
Amount	100 ul
Category	Neuroscience Neural Lineage Markers Cell Biology Cell Status Apoptosis Immunohistochemistry Western Blot Immunofluorescence Microglia Markers
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Alias	Apoptosis-inducing factor 1,mitochondrial,1.1.1.-,Programmed cell death protein 8,AIFM1,AIF,PDCD8
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Molecular Weight	66901 MW
Clonality	Polyclonal
Application Details	WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200
Gene Name	AIFM1
Gene Full Name	Apoptosis-inducing factor 1, mitochondrial
Immunogen	Synthetic peptide, corresponding to amino acids 61-110 of Human AIF-M1.
Contents	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Concentration	1 mg/ml
Protein Name	Apoptosis-inducing factor 1, mitochondrial
Protein Function	Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Subcellular Localization	Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
Description	Boster Bio Anti-AIF-M1 (Y85) Antibody catalog # A01571Y85. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A01571Y85-datasheet.pdf

BOS-A01571Y85-safety-datasheet.pdf