Anti-Phospho-PKC beta (S661) PRKCB Antibody

Primary Antibodies

76869 MW

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200

Protein kinase C beta type

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Calcium-activated, phospholipid- and diacylglycerol
(DAG)-dependent serine/threonine-protein kinase involved in
various cellular processes such as regulation of the B-cell
receptor (BCR) signalosome, oxidative stress-induced apoptosis,
androgen receptor-dependent transcription regulation, insulin
signaling and endothelial cells proliferation. Plays a key role in
B-cell activation by regulating BCR-induced NF-kappa-B activation.
Mediates the activation of the canonical NF-kappa-B pathway
(NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559',
'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1
association with lipid rafts and recruitment of the BCL10-MALT1
complex as well as MAP3K7/TAK1, which then activates IKK complex,
resulting in nuclear translocation and activation of NFKB1. Plays
a direct role in the negative feedback regulation of the BCR
signaling, by down-modulating BTK function via direct
phosphorylation of BTK at 'Ser-180', which results in the
alteration of BTK plasma membrane localization and in turn
inhibition of BTK activity. Involved in apoptosis following
oxidative damage: in case of oxidative conditions, specifically
phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to
mitochondrial accumulation of p66Shc, where p66Shc acts as a
reactive oxygen species producer. Acts as a coactivator of
androgen receptor (ANDR)-dependent transcription, by being
recruited to ANDR target genes and specifically mediating
phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag
for epigenetic transcriptional activation that prevents
demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In
insulin signaling, may function downstream of IRS1 in muscle cells
and mediate insulin-dependent DNA synthesis through the RAF1-
MAPK/ERK signaling cascade. May participate in the regulation of
glucose transport in adipocytes by negatively modulating the
insulin-stimulated translocation of the glucose transporter
SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is
probably involved in the inhibition of the insulin gene
transcription, via regulation of MYC expression. In endothelial
cells, activation of PRKCB induces increased phosphorylation of
RB1, increased VEGFA-induced cell proliferation, and inhibits
PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by
insulin, which causes endothelial dysfunction. Also involved in
triglyceride homeostasis (By similarity). Phosphorylates ATF2
which promotes cooperation between ATF2 and JUN, activating
transcription.

Boster Bio Anti-Phospho-PKC beta (S661) PRKCB Antibody catalog # A01940S661. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.