Item no. |
BOS-A05278-1-10ug |
Manufacturer |
Boster
|
Amount |
10 ug |
Format |
Lyophilized |
Applications |
WB, FC |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
Isotype |
IgG |
Citations |
1. Charbonneau, A., Luu-The, V. Assignment of steroid 5-beta-reductase (SRD5B1) and its pseudogene (SRD5BP1) to human chromosome bands 7q32-q33 and 1q23-q25, respectively, by in situ hybridization. Cytogenet. Cell Genet. 84: 105-106, 1999. 2. Charbonneau, A., The, V.-L. Genomic organization of a human 5-beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme. Biochim. Biophys. Acta 1517: 228-235, 2001. 3. Clayton, P. T., Mills, K. A., Johnson, A. W., Barabino, A., Marazzi, M. G. Delta 4-3-oxosteroid 5 beta-reductase deficiency: failure of ursodeoxycholic acid treatment and response to chenodeoxycholic acid plus cholic acid. Gut 38: 623-628, 1996. |
Alias |
3-oxo-5-beta-steroid 4-dehydrogenase; Aldo-keto reductase family 1 member D1; Delta (4)-3-ketosteroid 5-beta-reductase; Delta (4)-3-oxosteroid 5-beta-reductase; AKR1D1; SRD5B1 |
Available |
|
Manufacturer - Category |
Primary Antibodies |
Manufacturer - Isotype |
Rabbit IgG |
Storage Conditions |
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Observed Molecular Weight |
37 kDa |
Calculated Molecular weight |
39411 MW |
Clonality |
Polyclonal |
Application Details |
Western blot, 0.1-0.5?g/ml
Flow Cytometry(Fixed), 1-3?g/1x106 cells |
Manufacturer - Gene Name |
AKR1D1 |
Gene Full Name |
aldo-keto reductase family 1 member D1 |
Manufacturer - Specificity |
No cross reactivity with other proteins. |
Background |
Human delta (4)-3-oxosteroid 5-beta-reductase (steroid 5-beta-reductase) catalyzes 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta (4)-3-one structure. This gene is mapped to 7q33. The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta (4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1D1, which shares 90.9% and 93.9% amino acid (aa) sequence identity with mouse and rat AKR1D1, respectively. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
Purification |
Immunogen affinity purified. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. |
Manufacturer - Research Category |
Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Cancer, Cardiovascular, Lipid and Lipoprotein Metabolism, Cancer Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolism of Lipids and Lipoproteins, Cholesterol Metabolism, Lipids/Lipoproteins |
Protein Name |
Zinc finger protein Helios |
Protein Function |
Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha, 12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates. |
Subcellular Localization |
Cytoplasm. |
Description |
Boster Bio Anti-AKR1D1 Antibody Picoband® catalog # A05278-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. |
Tissue Specificity |
Highly expressed in liver. Expressed in testis and weakly in colon. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.