Anti-Parkin (phospho-S131) PRKN Antibody

Primary Antibodies

51641 MW

WB: 1:500-1:1000

E3 ubiquitin-protein ligase parkin

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Functions within a multiprotein E3 ubiquitin ligase
complex, catalyzing the covalent attachment of ubiquitin moieties
onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37,
RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30,
ZNF746 and AIMP2 (PubMed:10973942, PubMed:10888878,
PubMed:11431533, PubMed:12150907, PubMed:12628165,
PubMed:16135753, PubMed:21376232, PubMed:23754282,
PubMed:23620051, PubMed:24660806, PubMed:24751536). Mediates
monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked
and 'Lys-63'-linked polyubiquitination of substrates depending on
the context (PubMed:19229105, PubMed:20889974, PubMed:25621951).
Participates in the removal and/or detoxification of abnormally
folded or damaged protein by mediating 'Lys-63'-linked
polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-
linked polyubiquitinated misfolded proteins are then recognized by
HDAC6, leading to their recruitment to aggresomes, followed by
degradation (PubMed:17846173, PubMed:19229105). Mediates 'Lys-63'-
linked polyubiquitination of a 22 kDa O-linked glycosylated
isoform of SNCAIP, possibly playing a role in Lewy-body formation
(PubMed:11590439, PubMed:11431533, PubMed:19229105,
PubMed:11590439, PubMed:15728840). Mediates monoubiquitination of
BCL2, thereby acting as a positive regulator of autophagy
(PubMed:20889974). Promotes the autophagic degradation of
dysfunctional depolarized mitochondria (mitophagy) by promoting
the ubiquitination of mitochondrial proteins such as TOMM20,
RHOT1/MIRO1 and USP30 (PubMed:19029340, PubMed:19966284,
PubMed:23620051, PubMed:24896179, PubMed:25527291). Preferentially
assembles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin
chains following mitochondrial damage, leading to mitophagy
(PubMed:25621951). Mediates 'Lys-48'-linked polyubiquitination of
ZNF746, followed by degradation of ZNF746 by the proteasome;
possibly playing a role in the regulation of neuron death
(PubMed:21376232). Limits the production of reactive oxygen
species (ROS). Regulates cyclin-E during neuronal apoptosis. In
collaboration with CHPF isoform 2, may enhance cell viability and
protect cells from oxidative stress (PubMed:22082830).
Independently of its ubiquitin ligase activity, protects from
apoptosis by the transcriptional repression of p53/TP53
(PubMed:19801972). May protect neurons against alpha synuclein
toxicity, proteasomal dysfunction, GPR37 accumulation, and
kainate-induced excitotoxicity (PubMed:11439185). May play a role
in controlling neurotransmitter trafficking at the presynaptic
terminal and in calcium-dependent exocytosis. May represent a
tumor suppressor gene.

Boster Bio Anti-Parkin (phospho-S131) PRKN Antibody catalog # A31800S131. Tested in WB applications. This antibody reacts with Human.