Human CD11b ELISA Kit PicoKine®

PicoKine® ELISA Kits

ITGAM

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Human CD11b PicoKine® ELISA Kit (96 Tests). Quantitate Human CD11b in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1, 250 | 2, 500 | 5, 000 | 10, 000
O.D. | 0.051 | 0.109 | 0.190 | 0.415 | 0.839 | 1.341 | 1.749 | 2.052

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 796 | 785 | 804 | 812 | 799 | 20.78 | 2.6%
Sample 2 | 2799 | 2647 | 2860 | 2971 | 2819 | 87.4 | 3.1%
Sample 3 | 6548 | 6972 | 7069 | 7798 | 7097 | 283.87 | 4%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.