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Human Jagged1 ELISA Kit PicoKine®

Human Jagged1 ELISA Kit PicoKine®

Item no.	BOS-EK2224
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology By Cell Type Carcinoma ELISA Immunostaining
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<30 pg/ml
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	JAG1
Manufacturer - Specificity	Natural and recombinant human JAG1
Background	The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
Immunogen	Expression system for standard: CHO; Immunogen sequence: S32-S1046
Description	Human Jagged1 PicoKine® ELISA Kit (96 Tests). Quantitate Human Jagged1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 62.5 \| 125 \| 250 \| 500 \| 1, 000 \| 2, 000 \| 4, 000 O.D. \| 0.049 \| 0.072 \| 0.129 \| 0.218 \| 0.421 \| 0.775 \| 1.478 \| 2.079
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 90 \| 185 \| 1704 \| 76 \| 200 \| 1687 Standard deviation \| 2.79 \| 12.91 \| 48.39 \| 4.92 \| 10.12 \| 69.34 CV(%) \| 3.1% \| 7% \| 2.8% \| 6.5% \| 5.1% \| 4.1%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 90 \| 96 \| 75 \| 67 \| 82 \| 4.44 \| 5.4% Sample 2 \| 185 \| 143 \| 239 \| 169 \| 184 \| 12.6 \| 6.9% Sample 3 \| 1704 \| 1270 \| 1116 \| 1693 \| 1446 \| 93.40 \| 6.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	a polyclonal antibody from goat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EK2224-datasheet.pdf

BOS-EK2224-safety-datasheet.pdf